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Table 1 Performance of commercial reagents and platforms to detect hIL-33

From: Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development

Assay Platform Assay criteria
LLOQ in buffer (pg/mL) LLOQ in VH (pg/mL) sST2 tolerance (ng/mL) Immunodepletion (%) Sample volume (µL)
R&D Human IL-33 Quantikine Kit ELISA 6.25 62.5 1 n/a 50
Biolegend LEGEND MAX™ Human IL-33 Kit 15.6 n/a 10 n/a 50
Invitrogen
IL-33 Human Kit
1.6 3.2 1 81 50
Rb xhIL-33 (Pepro)/Biotin-goat xhIL-33 (R&D) 0.8 8 0.25 n/a 25
Mu xhIL-33 (Enzo)/Biotin-mu xhIL-33 (Novus) 20 n/a 0.5 59 25
U-PLEX Human IL-33
MSD Kit
ECL 0.6 2.4 0.5 60 25
Bio-Plex Pro™ Human Th17 Cytokine 15-Plex Kit Luminex 1.3 2.6 0.016 n/a 50
Mu xhL-33 (Enzo)/Biotin-mu xhIL-33 (Novus) Quanterix 20 n/a n/a n/a 150
  1. Parameters that did not meet our assay criteria selection are highlighted in bold; n/a represents parameters not tested