Table 1 Performance of commercial reagents and platforms to detect hIL-33

R&D Human IL-33 Quantikine Kit

ELISA

6.25

62.5

1

n/a

50

Biolegend LEGEND MAX™ Human IL-33 Kit

15.6

n/a

10

n/a

50

Invitrogen

IL-33 Human Kit

1.6

3.2

1

81

50

Rb xhIL-33 (Pepro)/Biotin-goat xhIL-33 (R&D)

0.8

8

0.25

n/a

25

Mu xhIL-33 (Enzo)/Biotin-mu xhIL-33 (Novus)

20

n/a

0.5

59

25

U-PLEX Human IL-33

MSD Kit

ECL

0.6

2.4

0.5

60

25

Bio-Plex Pro™ Human Th17 Cytokine 15-Plex Kit

Luminex

1.3

2.6

0.016

n/a

50

Mu xhL-33 (Enzo)/Biotin-mu xhIL-33 (Novus)

Quanterix

20

n/a

n/a

n/a

150