Fig. 2
From: SIRT5 functions as a tumor suppressor in renal cell carcinoma by reversing the Warburg effect
SIRT5 desuccinylates PDHA1. A Flag-PDHA1 and HA-SIRT5 were transfected into HEK293T cells, and the pansuccinylation level of PDHA1 was determined. B HA-tagged PDHA1 and Flag-tagged SIRT5 were coexpressed in HEK293T cells. PDHA1 copurified with SIRT5 was detected using an HA antibody. C HA-tagged SIRT5 and Flag-tagged PDHA1 were coexpressed in HEK293T cells. SIRT5 copurified with PDHA1 was detected using an HA antibody. D Diluted succinyl-CoA was added to the purified PDHA1 system, and the pansuccinylation level of recombinant PDHA1 (rPDHA1) was determined. E The pBABE-SIRT5 and control vectors were introduced into HeLa cells, and pansuccinylation of PDHA1 was determined. F SIRT5 was knocked down in HeLa cells, and pansuccinylation of PDHA1 was determined