Fig. 1

HFD increased CD226 expression in ATMs. A Comparison of soluble CD226 levels in sera of normal, overweight and obese individuals. Plots show individual data values obtained (79 individuals in total). Horizontal black lines represent median ± IQR. Differences between groups were determined by Kruskal–Wallis test. B The expression profiles of CD226 on myeloid cells, including monocytes, macrophages, and neutrophils. C, D FACS analysis of CD226 expression on macrophages in the spleen and epididymal SVF of C57BL mice fed with chow or a HFD for 16 weeks (n = 3). E Immunofluorescence staining for F4/80 (red) and CD226 (green) in the epididymal fat of mice fed with chow or a HFD for 16 weeks (n = 3). Scale bar means 100 μm. Data represent mean ± SEM. Differences between groups were determined by two-tailed, unpaired Student’s t-test. *P < 0.05, **P < 0.01