Fig. 10

The overexpression of circNOLC1 rescues propofol-attenuated proliferation and cancer stem cell activity of breast cancer. A–E The MDA-MB-231 and MDA-MB-468 cells were treated with propofol with or without circNOLC1 overexpressing plasmid. A and B The cell viability was detected by CCK-8 assays. C The sphere formation ability was determined by sphere formation assays. D and E The SP ration was identified by Hoechst 33342 staining using flow cytometry analysis. F–H The effect of propofol/circNOLC1 axis on tumor growth of MDA-MB-231 cells was analyzed by tumorigenicity analysis in nude mice. The tumor weight (F), Ki-67 levels (G), and STAT3 levels were shown. I A model of this study was shown, in which circNOLC1 contributed to CSCs properties and progression of breast cancer by targeting miR-365a-3p /STAT3 axis and propofol inhibited circNOLC1 by repressing STAT3 in a feedback mechanism. **P < 0.01