Fig. 4From: Circular RNA circPIP5K1A contributes to cancer stemness of osteosarcoma by miR-515-5p/YAP axisMiR-515-5p is a target of circPIP5K1A in osteosarcoma cells. A The interacted binding site between circPIP5K1A and miR-515-5p. B–D MG63 and 143B cells were transfected with miR-515-5p mimic. B MiR-515-5p expression was measured using qPCR. C, D Luciferase activities of circPIP5K1A or circPIP5K1A mutant (miR-515-5p binding site) were analyzed by luciferase reporter gene assay. E, F MiR-515-5p expression was determined using qPCR in MG63 and 143B cells transfected with circPIP5K1A siRNA. Mean ± SD (**P < 0.01)Back to article page