Fig. 6
From: miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer
CRLF1 attenuates the promotion effect of stemness induced by miR-3065-3p in CRC cells. a, b miR-3065-3p-overexpressing HCT116 cells or control cells (NC) were transfected with vector control (MCS) or CRLF1 plasmid for 24 h. (a) RT-qPCR analysis of CRLF1 expression in HCT116 cells. (b) Representative images of the wounding healing assay (up) and statistical results of wound healing area (down) were shown. c Transwell assay of migrated cells (up) and statistical results (down) of the indicated groups. d–f miR-3065-3p-overexpressing HCT116 cells or control cells (NC) were transfected with vector control (MCS) or CRLF1 plasmid for 48 h. d Flow cytometric analysis of ALDH activity (up) and its statistical results (down) were shown. RT-qPCR (e) and western blot (f, g) analysis of NANOG, OCT4, and SOX2 expression in HCT116 cells. The data shown represent the mean values ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001