Fig. 3

miR-3065-3p promotes the tumorigenesis and metastasis of colorectal cancer in vivo. a, b A mouse model of colorectal cancer with five mice in each group was established via orthotopic implantation of HCT116 cells overexpressing miR-3065-3p or negative control (NC). Images showing tumor size (a) and statistical results of tumor volume (b) are shown. A liver metastasis mouse model of colorectal cancer with five mice in each group was established via spleen injection of miR-3065-3p-overexpressing HCT116 or negative control (NC) cells. c, d Metastatic nodules of the liver (c) and representative images of HE-stained metastatic lesions (d) are shown. e, f Western blot (e) and immunohistochemical (f) analysis of NANOG, OCT4, and SOX2 expression in tumor tissue from xenograft mice. ‘1–4’ represented the different four mice in the same group. Statistical results are shown in the right panel. The data shown represent the mean values ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001