Fig. 4

STAT4 promotes Runx3 expression by mediating the level of H3K4me3. A Binding site of STAT4 in Runx3 promoter region predicted by JASPAR. B ChIP-qPCR detected the binding of STAT4 in Runx3 promoter region. * p < 0.05 vs. the Normal IgG group. C IHC assay of Runx3-positive ratio in rat aortic tissues and corresponding quantification (× 400); * p < 0.05 vs. the Con group. D Western blot analysis of Runx3 protein expression in HUVECs under environment of Con and HG. * p < 0.05 vs. the Con group. E ChIP-qPCR of relative H3K4me3 and STAT4 recruitment level in the Runx3 promoter region of aortic tissues from rat aortic tissues. * p < 0.05 vs. the IgG group, # p < 0.05 vs. the Con group. F RT-qPCR analysis of Runx3 expression in HUVECs after treatment with Con + sh-STAT4-1, Con + sh-STAT4-2, Con + oe-STAT4 or controls. * p < 0.05 vs. the Con + oe-NC group and # p < 0.05 vs. the Con + sh-NC group. G ChIP-qPCR of relative H3K4me3 and STAT4 recruitment level in HUVECs under Con treatment and transfection with sh-STAT4-1, oe-STAT4 or controls. * p < 0.05 vs. the Con + oe-NC group and # p < 0.05 vs. the Con + sh-NC group. Data were measurement data, and presented as mean ± standard deviation. The data between two groups was analyzed by unpaired t-test. Data among groups were analyzed by one-way ANOVA. Experiment was repeated three times independently