Fig. 3

CircSERPINA3 stabilizes SERPINA3 via recruiting BUD13. A RIP assay was used to examine the enrichment of circSERPINA3 and SERPINA3 in PC-3 cells incubated with different antibodies of candidate shared RBP screened out from starBase. B, C Western blot analysis was conducted to examine BUD13 expression when circSERPINA3 was down-regulated or overexpressed in PC-3 cells. D, E RT-qPCR was used to examine the knockdown efficiency of sh-BUD13-1/2/3 and overexpression efficiency of pcDNA3.1-BUD13 in PCa cells. F, G RT-qPCR was carried out to examine SERPINA3 expression when BUD13 was reduced or overexpressed in PC-3 cells. H, I RT-qPCR was used to examine the effect of sh-BUD13-1 on half-life of SERPINA3 and β-actin in PC-3 cells. J, K Expression of SERPINA3 and β-actin in PC-3 cells was tested by means of RT-qPCR after cells were transfected with pcDNA3.1-BUD13. (sh short hairpin, NC negative control). ^**P < 0.01