Fig. 2

UBE2T upregulates autophagy in NSCLC cells. A–D Detection of autophagy in the labeled cells under starvation at different time points. Cells were starved in EBSS for 8 h to induce autophagy prior to analysis. Cell lysis was collected, and western blot was used to detect markers of autophagy. E, G Overexpression of UBE2T stimulated autophagy. F–H Silencing of UBE2T inhibited autophagy. I, J UBE2T overexpression increased autophagy in H1299 cells. GAPDH was used as internal controls. All experiments were repeated at least three times. Protein bands were quantified using Image J. *P < 0.05, **P < 0.01, ***P < 0.001