Fig. 3

Circ_0001361 sponges miR-525-5p in LUAD cells. A The relationship between miR-525-5p and circ_0001361 was verified by dual luciferase assay. B RIP assay assessed the interaction between circ_0001361 and miR-525-5p in A549 cells. C RNA pull-down assay was performed in A549 cells using biotinylated miR-525-5p probe, and subsequent RT-qPCR for determining the enrichment of circ_0001361. D RT-qPCR for the expression of miR-525-5p in LUAD and normal adjacent tissues. E The correlation between circ_0001361 and miR-525-5p was analyzed. F The level of miR-525-5p in normal human bronchial epithelial cells and LUAD cells was determined by RT-qPCR. G The expression of miR-525-5p in A549 and PC9 cells after transfection with sh-circ_0001361 was evaluated by RT-qPCR. All data are expressed as the means ± standard deviation from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus the indicated group