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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Knockdown of long noncoding RNA GAS5 reduces vascular smooth muscle cell apoptosis by inactivating EZH2-mediated RIG-I signaling pathway in abdominal aortic aneurysm

Fig. 4

GAS5 promotes SMC apoptosis partially by downregulating EZH2 expression. A The interaction between GAS5 and EZH2 analyzed by RIP assay. B The expression of GAS5 and EZH2 in SMCs transfected with oe-GAS5 or sh-GAS5 detected by RT-qPCR. C Western blot analysis of EZH2 protein in SMCs transfected with oe-GAS5 or sh-GAS5. D Transfection efficiency of GAS5 and EZH2 in SMCs determined by RT-qPCR. E Cell apoptosis in Ang II-induced SMCs co-treated with oe-EZH2, oe-NC and oe-GAS5 or control mice treated with oe-NC measured by flow cytometry. F Western blot analysis of cleaved caspase-3, Bcl-2 and Bax proteins in Ang II-induced SMCs co-treated with oe-EZH2, oe-NC and oe-GAS5 or control mice treated with oe-NC. β-actin served as internal reference of EZH2 and cleaved-caspase-3. *p < 0.05 indicates significant difference. Data in B and C were compared using unpaired t-test while in DF by one-way ANOVA with Tukey’s post-hoc tests. Cell experiments were repeated three times independently

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