Fig. 6

FGF11 regulates HIF-1α expression for NSCLC progression. A Gene Set Enrichment Analysis (GSEA) of the hypoxia signaling pathway genes in FGF11 positive and negative samples usingGSE81089 RNA-seq data. B qPCR analysis of HIF-1α mRNA expression after FGF11 knockdown in A549 and NCI-H460 cells. C Western blot analysis of HIF-1α protein level after FGF11 knockdown in A549 and NCI-H460 cells. D qPCR analysis of HIF-1α mRNA expression in A549 and NCI-H460 cells with FGF11 overexpression. E Western blot analysis of HIF-1α protein level in A549 and NCI-H460 cells with FGF11 overexpression. F qPCR quantification of HIF-1α mRNA level after siRNA transfection. G CCK-8 proliferation assay in A549 and NCI-H460 cells transfected with FGF11 and/or HIF-1α siRNA. H Colony formation assay in A549 and NCI-H460 cells transfected with FGF11 and/or HIF-1α siRNA. I wound healing assay in A549 and NCI-H460 cells transfected with FGF11 and/or HIF-1α siRNA. J Transwell migration assay in A549 and NCI-H460 cells transfected with FGF11 and/or HIF-1α siRNA. The results are represented as mean ± standard derivation. Data are summary of 3 independent experiments. *P < 0.05, ** P < 0.01, ***P < 0.005 vs. sh-NC or empty vector-transfected cells