Fig. 2

Knockdown of TRIM29 expression suppressed the growth of pancreatic cancer cells in vitro and in vivo. A and B, qRT-PCR and western blot analysis of TRIM29 expression in pancreatic cancer (PC) cell lines (SW1900, PANC-1, AsPC-1, and BxPC-3) and a normal human pancreatic ductal epithelial cell line HPDE6-C7 (*p < 0.05, **p < 0.01). C and D, after transfection with shTRIM29 plasmids, qRT-PCR and western blotting showed that the level of TRIM29 was reduced (**p < 0.01). E and F, CCK8 and EdU assays showed that shTRIM29 cell proliferation was inhibited (*p < 0.05). G and H, BxPC-3/shTRIM29 and BxPC-3/shNC cells were injected subcutaneously into nude mice, and the tumour volumes were measured on the indicated days. At the end of the experiment, the tumours were dissected, photographed, and weighed (n = 5, * p < 0.05). I IHC assay detected the expression of BxPC-3/shTRIM29 and BxPC-3/shNC