Fig. 7

BCLAF1 phosphorylation at Ser290 is involved in DNA damage repair and promotes IR-induced DNA damage responses. a HGC cells were treated with 0, 5, 10 or 15 Gy IR for 2 h. Western blot was performed to detect pBCLAF1 (Ser290) and BCLAF1. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. 0 Gy. b HGC cells were exposed to IR (0, 5, 10 or 15 Gy) for 2 h. Cells were double-stained with monoclonal antibody to γ-H2AX and polyclonal antibody to pBCLAF1 (Ser290). IR-induced pBCLAF1 (Ser290) foci were colocalized with γ-H2AX foci. Scale bars, 100 µm. c HGC and AGS stably cells overexpressing WT, S290D, S290A forms of BCLAF1 were treated with IR (15 Gy for 2 h), γ-H2AX level was determined using immunofluorescence. Scale bars, 100 µm. Numbers of γ-H2AX foci per cell were quantified and statistically analyzed by Student's t-test. *p < 0.05 and **p < 0.01 vs. WT + 15 Gy, ^#p < 0.05 vs. EV + 15 Gy