Fig. 2

Cldn7 knockdown promoted the TIC features and biological behavior of CRC stem cells. a HCT116^CD133+CD44+ shCldn7 cells formed tumour spheres faster than HCT116^CD133+CD44+ shcontrol cells. b Both cell spheres returned to an adherent state in complete medium, and the cells dissociated from the cell sphere and continued to grow and proliferate. c Tumour growth curves showed that the tumour formation rate and tumour volume formed by HCT116^CD133+CD44+shCldn7 cells were significantly greater than control cells (P < 0.05). d Proliferation ability of HCT116^CD133+CD44+shCldn7 cells was increased (P < 0.05). e Apoptosis ability of HCT116^CD133+CD44+shCldn7 cells was weakened (P < 0.05). f Migration ability of HCT116^CD133+CD44+shCldn7 cells was greater than control cells (P < 0.05)