Fig. 4
From: SRPK1/AKT axis promotes oxaliplatin-induced anti-apoptosis via NF-κB activation in colon cancer
SRPK1 increased the phosphorylation of AKT to activate the NF-κB pathway. a Western blotting analysis the phosphorylation of AKT in SW480 and HCT-116 cells overexpressing SRPK1 or vector only, and downregulated for SRPK1, or vector only. b Luciferase assay to determine the transcriptional activity of NF-κB in SW480 and HCT-116 cells overexpressing, or silenced for, SRPK1, and vector only. c qPCR analysis of genes downstream of the NF-κB pathway in SW480 and HCT-116 cells overexpressing SRPK1 or vector only, and downregulated for SRPK1, or vector only. The bars indicate the means ± SD (n = 3). d Western blot analysis of the NF-κB p65 subunit in the nuclear and cytoplasmic fractions of SW480 and HCT-116 cells overexpressing SRPK1, or vector only, and downregulated for SRPK1, or vector only. e, Immunofluorescence analysis of p65 in SW480 and HCT-116 cells overexpressing SRPK1, or vector only, and downregulated for SRPK1, or vector only. SRPK1 Serine-arginine protein kinase 1, AKT protein kinase B, NF-κB nuclear factor kappa B, qPCR quantitative real-time polymerase chain reaction