Fig. 3

Upregulated SRPK1 promotes the ability of anti-apoptosis in colon cancer cell lines. a SRPK1 overexpression and downregulation in SW480 and HCT-116 cells were confirmed using western blotting. b MTT assay was examined and the IC₅₀ was calculated in SW480 and HCT-116 cells overexpressing, or silenced for, SRPK1 and vector only with increasing oxaliplatin exposure (n = 6). c Representative TUNEL staining images of the indicated cells treated with increasing oxaliplatin concentrations, quantification of TUNEL⁺ cells in SW480 and HCT-116 cells overexpressing, or silenced for, SRPK1, and vector only with oxaliplatin treatment (n = 3). d Levels of Bcl-xL, Bcl-xS, and cleaved PARP1 in SW480 and HCT-116 cells overexpressing, or silenced for, SRPK1, and vector. β-tubulin was used as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001. SRPK1 Serine-arginine protein kinase 1, TUNEL terminal deoxynucleotidyl transferase nick-end-labeling, Bcl-xL BCL2 Like 1 long isoform, Bcl-xS BCL2 Like 1 short isoform, PARP1 poly(ADP-ribose) polymerase 1