Fig. 2

MiR-15b-5p regulates HRVECs function through targeting COL12A1. A A Veen diagram reveals the predicted target genes of miR-15b-5p from TargetScan, Star Base and microRNA.org. B For the 6 screened target genes, qRT-PCR was used to detect the expression difference induced by HG. C Luciferase reporter vectors containing the wild-type or mutant 3′ UTR of the COL12A1 were constructed according to the predicted binding sites of miR-15b-5p. D The histogram shows the changes of luciferase activities under different conditions. The HRVECs were co-transfected with luciferase reporter vector and miR-15b-5p mimics or mimics control. The expression of COL12A1 in HRVECs induced by HG was detected by qRT-PCR analysis (E) and western blot assay (F). G Wound healing assay was used to detect the invasion of HRVECs in different groups. H Transwell assay was used to detect the migration of HRVECs in different groups. I Tube formation experiment was performed to assess the tubule-forming ability of cells in different groups. The data was presented as mean ± SEM, n = 3 each group. Vs control/HG + mimics control group, *P < 0.05, **P < 0.01; vs HG + miR-15b-5p mimics + pcDNA3.1 group, ^#P < 0.05, ^##P < 0.01