Fig. 4

Knockdown of STAT3 alleviated Met-5A cells EMT and HIF-1α expression induced by high glucose. (A) Met-5A cells were seeded and then transfected with siRNA against STAT3 (100 nM) or negative control (100 nM) and 12 h later, cells were incubated with high glucose (60 mmol/L) for 48 h. (B-E) Expression of HIF-1α, Fibronectin, α-SMA, STAT3 in cells by western blot analysis. GAPDH was used as a loading control. The data were the means ± SE of three experiments. P values were determined by t tests. *P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant