Fig. 1
From: STAT3/HIF-1α signaling activation mediates peritoneal fibrosis induced by high glucose
The level of phosphorylated STAT3 of HPMCs ex vivo in drained PD effluent. A HPMCs were cultured from drained PD effluent of two patients (patient 1: PD duration 1 month; patient 3: PD duration 3 years). Then cellular localization of p-STAT3 (green) in HPMCs was detected using immunofluorescence analysis. Original magnification, ×200. B Western blot was performed with PD effluent cell lysates centrifuged from patients with different PD duration. The levels of Fibronectin and p-STAT3 were present in these long-term patients (No. 2, 3, 4; PD duration 1 year, 3 years and 6 years, respectively) but not in a new patient (No. 1; PD duration 1 month)