Fig. 6

ELK4 promotes M2 polarization of macrophages by regulating KSR1 to promote the proliferation, migration and invasion of GC cells. A Western blot was used to detect the expression of ELK4, KDM5A, PJA2 and KSR1 in TAMs. B mRNA and protein expression of M1 and M2 macrophage markers in TAMs cells was detected by RT-qPCR and ELISA. C The proliferation of HGC-27 cells was detected by CCK-8 assay after TAMs were co-cultured with HGC-27. D After TAMs were co-cultured with HGC-27 cells, the migration and invasion of HGC-27 cells were detected by Transwell assay (× 200). *p < 0.05