Fig. 5

PJA2 decreases KSR1 by ubiquitination, thus suppressing macrophage M2 polarization. A The expression of KSR1 in GC data collected by TCGA and GTEX (T: Tumor. N: normal. *p < 0.05). B KSR1 protein expression level in GC tissues and adjacent tissues of GC patients was detected by Western blot (n = 30). C KSR1 protein expression levels in TAMs in GC tissues and in Macs of adjacent tissues was detected by Western blot. D After overexpression of PJA2 in TAMs, Western blot was used to detect the protein expression of KSR1. E After overexpression of PJA2 in TAMs, the binding of KSR1 to ubiquitin was detected by co-IP assay. F The stability of KSR1 protein was detected by CHX treatment after overexpression of PJA2 in TAMs. G The mRNA and protein expression levels of M1 and M2 macrophage markers in TAMs cells were detected by RT-qPCR and ELISA. *p < 0.05