Fig. 2

FOXP3 is negatively targeted by miR-149-3p in EC. A The binding sites between miR-149-3p and FOXP3 predicted by Starbase. B The targeting relationship between miR-149-3p and FOXP3 evaluated by dual luciferase reporter assay. * p < 0.05 vs. the treatment of NC-mimic. C miR-149-3p expression in cancer and paracancerous tissues detected by RT-qPCR normalized to U6 (n = 56). * p < 0.05 vs. paracancerous tissues. D The correlation between miR-149-3p and FOXP3 expression in EC tissues. E Expression of miR-149-3p in AKR cells detected by RT-qPCR normalized to U6. F Protein expression of FOXP3 in AKR cells measured by western blot analysis normalized to GAPDH. * p < 0.05 vs. control cells. G Protein expression of FOXP3 in different AKR cells measured by western blot analysis normalized to GAPDH. * p < 0.05 vs. AKR cells treated with NC-mimic. # p < 0.05 vs. AKR cells treated with NC-inhibitor. The measurement data were shown as mean ± standard deviation. Data between cancer and paracancerous tissues were compared by paired t test, and data between other two groups were compared by independent sample t test. Pearson was used for correlation analysis between miR-149-3p and FOXP3 expression in EC tissues. The cell experiment was repeated 3 times independently