Fig. 3

Immune characteristics of two subtypes in the training set and validation set. A Comparisons of the stromal scores, immune scores, and ESTIMATE scores for the two subtypes in all three cohorts. B Heatmap showing the abundance of immune cells for different subtypes in the SCCH cohort. The relative abundance of each cell type was standardized using zero-mean normalization. C The boxplot representation of immune cells with significant differences in distribution between the subtype 1 and subtype 2 in the SCCH cohort. D Evaluation of the level of 4 important immune scores and tumor mutational burden (TMB) between two subtypes in the training set and validation set. The statistical difference in A, C, D was compared through the Wilcoxon rank-sum test. (ns represents no significance, * p-value < 0.05, **p ≤ 0.01, *** p-value ≤ 0.001, **** p ≤ 0.0001). E Correlation between cytolytic activity (CYT), immune infiltration score (IIS), and T cell infiltration score (TIS) in three cohorts. F Correlation between cytolytic activity (CYT), immune infiltration score (IIS), T cell infiltration score (TIS), and TMB in the training set and validation set. The p-value and Pearson’s correlation coefficients with a 95% confidence interval shown at the top of each plot