Fig. 3
miR-20a suppresses Treg differentiation in EAE. A The mRNA levels of characteristic transcription factors of Treg (Foxp3), Th1 (Tbx21) and Th17 (Rorc) cells in CD4+ T cells of EAE and control mice. B The mRNA levels of characteristic cytokines of Treg (Il-10, Tgfb1), Th1 (Ifng) and Th17 (Il-17a, Il-17f) cells in CD4+ T cells of EAE and control mice. C The mRNA levels of Foxp3 in Treg-polarized CD4+ T cells with miR-20a or NC antagomirs transfected. D The mRNA levels of Il-10 in Treg-polarized CD4+ T cells with miR-20a or NC antagomirs transfected. E, F. The percentages of CD25+Foxp3+ cells in CD4+ T cells of EAE mice after a 5-day MOG35-55 stimulation and Treg polarization with miR-20a or NC antagomirs transfected. The flow cytometry plots shown are representative of 6 independent experiments. Data are shown as mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001 using unpaired (A–D) and paired (F) Student’s t-test