Fig. 8

eIF6 activated mTOR-related cancer signaling pathways in HCC progression. a The expression level of mTOR-related markers was determined by western blotting (up). The blot density of the western blotting was quantified using the ImageJ software. β-actin was used as the loading control (down). b Western blotting was used to measure the expression levels of cell cycle, apoptosis and invasion regulators, including MYC, CCND1, CDK4, CDK6, cleaved-CASP3 (cl-CASP3), pro-CASP3 (pr-CASP3) and CTNNBL1. The blot density of the western blotting in HepG2 cells (c) and Huh-7 cells (d) was quantified using the ImageJ software. e–i Analysis of the correlation coefficient between eIF6 and cell progression-related genes shown by western blot analysis using the R2 database (n = 373). j A working model by which eIF6 activated mTOR-related cancer signaling pathways in HCC progression. ^#p > 0.05; *p < 0.05; **p < 0.01; and ***p < 0.001