Table 1 Primer sequences used for quantitative RT-PCR
From: Cigarette smoke-associated inflammation impairs bone remodeling through NFκB activation
Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
Cat K | GTGTTGGTGGTGGGCTATG | GCAGGCGTTGTTCTTATTCC |
TRAP | CACCCTGAGATTTGTGGCTGT | CGGTTCTGGCGATCTCTTTG |
CTR | GGCGACTATCTACTGCTTCTG | GATTCCGTGGTTCCTGATGG |
Runx2 | TGGCTTGGGTTTCAGGTTAGGG | TCGGTTTCTTAGGGTCTTGGAGTG |
OCN | TAGTGAACAGACTCCGGCGCTA | TGTAGGCGGTCTTCAAGCCAT |
Col I | CTGACTGGAAGAGCGGAGAG | GCACAGACGGCTGAGTAGG |
OSX | AGAGGTTCACTCGCTCTGACGA | TTGCTCAAGTGGTCGCTTCTG |
BSP | AAGAGGAAGAAAATGAGAACGA | GCTTCTTCTCCGTTGTCTCC |
OPN | CCA ATG AAA GCC ATG ACC ACA | CGT CAG ATT CAT CCG AGT CCA C |
OPG | TACCTGGAGATCGAATTCTGCTT | CCATCTGGACATTTTTTGCAAA |
RANKL | GAAACTCACAGCCCTCTCTCTTG | GCATCGGAATACCTCTCCCAATC |
GAPDH | CAGTGCCAGCCTCGTCCCGTAGA | CTGCAAATGGCAGCCCTGGTGAC |