Skip to main content
Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Inhibition of DKC1 induces telomere-related senescence and apoptosis in lung adenocarcinoma

Fig. 4

DKC1 downregulation induced telomere-related cell senescence and apoptosis. a qPCR detecting the abundance of TERC and DKC1 in DKC1 knockdown A549 and PC-9 cells. b qPCR-based Telomerase Repeated Amplification Protocol (qTRAP) analyses for telomerase activity. The left panel shows the relative telomerase activity of DKC1 knockdown A549 and PC-9 cells (5000 cells, 500 cells and 50 cells). The right panel shows the gel image of TRAP assay on 5000 cells. c qPCR-based telomere length assessment of control and DKC1 silenced A549 and PC-9 cells. d Senescence-associated-β-galactosidase staining of control and DKC1 silenced A549 and PC-9 cells. Scale bar: 200 µm. e Immunoblot detecting DKC1, P21 and γH2A.X expression in control and DKC1 silenced A549 and PC-9 cells. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page