Fig. 3

KLF5 transcriptionally regulates Slug expression to promote airway inflammation and remodeling. a The binding of KLF5 in the Slug promoter in BEAS-2B and HBE cells as examined by ChIP assay. b The KLF5 transcriptional regulation on Slug as examined by dual luciferase reporter gene assay. c KLF5 and Slug expression as determined by RT-qPCR and Western blot assay. d The silencing effect of Slug as determined by RT-qPCR. E The Slug silencing effect of Slug expression as determined by Western blot assay. F The expression of KLF5 and Slug in BEAS-2B and HBE cells with different treatment as determined by RT-qPCR. g The expression of TGF-β and inflammatory factors IL-4, IL-5, and IL-13 in supernatant of BEAS-2B and HBE cells with different treatment as examined by ELISA. h The proliferation of BSMCs with different treatment as examined by CCK-8 assay. i The migration of BSMCs with different treatment as examined by Transwell assay. *p < 0.05 vs. IgG, vector, or sh-NC. #p < 0.05 vs. control. These data are measurement data, expressed as mean ± standard deviation. Data between two groups were compared using unpaired t-test. Data among multiple groups were compared using one-way ANOVA, combined with Tukey’s post-hoc tests