Fig. 7
Tumor volume and body weight changes in tumor-bearing mice. The tumor volume and body weight of tumor-bearing mice were measured every other day during the 14-day period of intragastric FYD administration. The mean tumor volume change of each group was recorded and calculated daily. a These figures showed the tumor-bearing mice and tumor. b A growth curve was drawn based on the tumor volume change in each group, which revealed the trend in tumor volume growth in each experimental group. The tumor volume growth in the FYD + IL-12 + BMSC group was significantly lower than that of the model and BMSC groups. For the comparison of tumor volume, the sphericity test P = 0.00 < 0.01, which did not conform to the sphericity, and the Greenhouse–Geisser model gives F = 482.8, P = 0.00, indicating that the main effect difference was statistically significant at different time points. The comparison of Tukey’s test between the two groups found that the difference between any two groups was statistically significant (P = 0.00); and the interaction test time ╳ group found F = 16.79, P = 0.00, indicating there is interaction with the group. In addition, the tests of between-subjects effects test found statistically significant differences in the main effects between the different groups (F = 28.22, P = 0.00). n = 3, *P < 0.05: On the 7th, 9th, 11th and 14th days, IL-12 + BMSCs group, FYD group, FYD + IL-12 + BMSCs group vs Model group, only except on the 9th and 11th day, IL-12 + BMSCs group vs Model group. #P < 0.05, On 14th day, with the exception of the FYD group, all other groups vs IL-12 + BMSCs group. ※P < 0.05: On 14th day, all other groups vs the FYD + IL-12 + BMSCs group. c The body weight of the FYD + IL-12 + BMSC group was significantly lower than that of all other groups. For the comparison of body weight changes in tumor-bearing mice, the sphericity test which does not conform to the sphericity (P = 0.00 < 0.01). There were statistically different in the main effect on time points with Greenhouse–Geisser model F = 13.73, P = 0.00. Interaction test time ╳ group found that there was interaction between time and group (F = 3.053, P = 0.02). In addition, there was a statistically significant difference in the main effect between the different groups on the 14th day by the tests of Between-Subjects Effects using Tukey’s test method (F = 75.779, P = 0.00). n = 3, *P < 0.05: On the 14th day, IL-12 + BMSCs group, FYD group, FYD + IL-12 + BMSCs group vs Model group, #P < 0.05: On the 14th day: all other groups vs all other groups vs IL-12 + BMSCs group, ※P < 0.05: All other groups vs the FYD + IL-12 + BMSCs group. Note: BMSCs, bone marrow mesenchymal stem cells; IL-12 + BMSC, IL12-lentiviral-transfected BMSC; BMSC group, empty lentiviral vector-transfected BMSC group; FYD, FuzhengYiliu decoction; FYD + IL-12 + BMSC group, FYD treatment in IL-12 lentiviral-transfected BMSC group