Fig. 5

CircPLXNB2 promoted tumour growth in AML xenograft models. a Images of tumour-bearing mice show that the tumours were larger in circPLXNB2 OE-treated mice and smaller in circPLXNB2 shRNA-treated mice. The tumour sizes (b) were observed for 28 days, the mice were sacrificed at the end of 28 days, and the weights (c) of subcutaneous tumours were also measured after 28 days. H&E and TUNEL staining of xenograft tumour tissues obtained from three mice randomly selected from each group (n = 6). Representative fields are shown. d H&E (upper panel) and TUNEL (lower panel) staining of the xenograft tumour tissues from mice treated with OCI-AML3 cells transfected with shRNA NC or circPLXNB2 shRNA. e H&E (upper panel) and TUNEL (lower panel) staining of the xenograft tumour tissues from mice treated with HL-60 cells transfected with vector or circPLXNB2 OE. In the H&E-stained sections, the original magnification was 200×. Positive cells are indicated by brown staining in the TUNEL-stained sections, with an original magnification of 400×. All parameters were measured in three separate experiments. The vector or shRNA NC was used as a control. *P < 0.05, ***P < 0.001, ^^P < 0.01, and ^^^P < 0.001. CircPLXNB2 OE circPLXNB2 overexpression, circPLXNB2 shRNA circPLXNB2 short hairpin RNA