Fig. 3

Knockdown of CMTM6 inhibits HCC cell proliferation and metastasis in vivo. a Images of mice injected with CMTM6-silenced MHCC-97H cells. b, c IHC and Ki-67 expression in tumours from mice injected with HCC cells. d Tumour weight of model mice; the tumours were lighter in the mice that received cells silenced for CMTM6 expression than in those that received cells transduced with the empty vector. Tumour weights are indicated by the data points. e Fluorescence images of metastatic lung nodules of mice injected with Bel-7402 cells silenced for CMTM6. f Representative H&E staining of lung metastasis nodules. g The number of lung metastatic loci. Student t test was used to analyse the number of metastatic nodules. h The expression of Ecadherin in tumors was determined using quantitative PCR (qPCR). **p < 0.001, vector vs. shCMTM6