Fig. 5

Target gene validation of miR-15a and miR-92a. Expression of selected target genes of a miR-15a and d miR-92a in T98 and U251 cell lines as analyzed by qRT-PCR assays. Protein expression of selected target genes of b miR-15a and e miR-92a in T98 and U251 cell lines as measured by western blot assays. Effects of c miR-15a and f miR-92a on the luciferase reporter activity of cells with CCND1 WT or CCND1 MUT and RAP1B WT or RAP1B MUT, respectively. Error bars denote standard deviation of triplicates. **P < 0.01; *P < 0.05. WT: wild type; MUT: mutant type