Fig. 3

Cytokine secretion and cytotoxic activity of the meso3 CAR-T and meso3-CD40 CAR-T cells. a Cytokine secretion from meso3 CAR-T and meso3-CD40 CAR-T cells was determined by a cytometric bead array after costimulation with the rhMSLN antigen. b The MSLN expression level in SKOV-3 cells was validated by flow cytometry. c The cytotoxicities of the meso3 CAR-T and meso3-CD40 CAR-T cells against SKOV3 cells were assessed using the RTCA system at low effector-to-target ratios (E:T = 1:2 and 1:4), and the mock T cells group was used as a negative control. d Quantification of the data of the specific cytotoxicities of CAR T cells against SKOV-3 cells at different E:T ratios (n = 3, mean ± SEM, ns, not significant, *P < 0.05; **P < 0.01; ***P < 0.001)