Fig. 4

PX inhibits LPS-induced ferroptosis and inflammation in vitro. BEAS-2B cells are treated with different concentrations of PX (10, 20, and 40 μg/ml) in the presence of LPS (10 μg/ml) for 24 h. Then cells and culture supernatants are collected used for ferroptosis and inflammation detection. a Cell viability determined by MTT assay. b Cell death detected by FDA staining kit. c Fe²⁺ level determined using Iron Assay Kit. d MDA level detected via the MDA Assay Kit. e GSH level detected by GSH assay kit. f GPX4 activity measured by GPX4 ELISA kit. g GPX4 mRNA expression detected by RT-PCR. h GPX4 protein expression detected by western blot assay. i TNF-α, IL-1β, and IL-6 level in cell culture supernatants determined via ELISA Kits. ^*P <0.05 vs. ctrl group, and ^#P <0.05 vs. LPS group