Fig. 1
From: Genome-wide analysis identifies critical DNA methylations within NTRKs genes in colorectal cancer
The flow diagram of cohort analyses. The Fred Hutchinson Cancer Research Center (FHCRC) cohort was an in-house retrospective cohort with 64 colorectal cancer (CRC) samples and 41 normal colon samples. The DNA methylation profile of FHCRC cohort was used to identify the differentially methylated probes (DMPs) within NTRKs genes between tumor and normal samples. We further used the clinical information, DNA methylation and mRNA expression profiles of TCGA-COAD & READ, GSE83889, GSE39582 and GSE87211 cohorts that prospectively enrolled patients with CRC to screen the critical methylated CpG sites within NTRKs genes. The SYSU (Sun Yat-sen University) cohort was an in-house retrospective cohort of 229 patients diagnosed with CRC that was used to validate the identified critical methylated CpGs. The external cohort is a set of 23 tumor types from TCGA, which was used to demonstrate the significance of identified critical methylated CpGs in pan-cancer