Fig. 4
CBX8 recruits PRC1-BCOR to the PRDM1 promoter region to downregulate PRDM1 expression. a The correlation analysis between expression of CBX8 and PRDM1 in BCa according to GEPIA (p = 0.00017). b The box plot of PRDM1 expression in BCa samples (left red box) and normal samples (right grey box) according to GEPIA. *p < 0.05 vs. normal samples analyzed by paired t-test. c The silencing efficiency of sh-CBX8 determined by Western blot analysis. d The enrichment of CBX8 in the PRDM1 promoter region detected by ChIP assay. e The interaction between CBX8 and BCOR detected by co-IP. f The enrichment of BCOR in the PRDM1 promoter region detected by ChIP. g The relative luciferase activity determined by dual luciferase reporter assay. h The expression of PRDM1 in response to CBX overexpression/knockdown determined by RT-qPCR. i The expression of PRDM1 in response to CBX overexpression/knockdown determined by Western blot analysis. *p < 0.05 vs. T24 cells treated with sh-NC (panels c, d, f and g) or T24 cells treated with oe-NC (panels h and i); #p < 0.05 vs. T24 cells treated with sh-NC (panels h and i)