Fig. 2

KPNA2 assists nucleus shuttling of CBX8. a The binding between KPNA2 and CBX8 in T24 and BIU-87 cells detected by Co-IP assay. b The PNA2 and CBX8 mRNA expression detected in T24 and BIU-87 cell lines using RT-qPCR. c The expression of KPNA2 and CBX8 in BCa cells in response to overexpressing/silencing KPNA2 determined by Western blot analysis. d The expression of CBX8 in the nucleus of BCa cells in response to overexpressing/silencing KPNA2 determined by Western blot analysis. e The expression of CBX8 in the cytoplasm of BCa cells in response to overexpressing/silencing KPNA2 determined by Western blot analysis. f The nucleus accumulation of KPNA2 and CBX8 in T24 cells by immunofluorescence staining. *p < 0.05 vs. T24 cells treated with oe-NC; ^#p < 0.05 vs. T24 cells treated with sh-NC