Fig. 4

LINC01116 recruits EZH2 to suppress TPM1 gene transcription. The binding of LINC01116 and EZH2 were predicted by RNAInter online tools (a). The potential binding of EZH2 and TPM1 was predicted by UCSC (b). TCGA database was used to assess the expression of EZH2 in CRC tissues and the association between EZH2 and TPM1 (c). qRT-PCR (d) and Western blot (e) were applied to measure the levels of EZH2 in CRC cell lines. RIP assay confirmed the interaction between LINC01116 and EZH2 (f). Binding of EZH2 to TPM1 promoter was verified by ChIP (g). The augment of TPM1 promoter enriched in EZH2 antibody following overexpression of LINC01116 was detected by ChIP (h). RNA pull-down assay was performed to measure H3K27me3 in LINC01116-complex following overexpression of LINC01116 (i). *P < 0.05, **P < 0.01, ***P < 0.001; TPM1 tropomyosin 1, CRC colorectal cancer