Fig. 4

IFN pathway activation by EGFR/ERBB inhibitor in murine B4B8 HNSCC cells. a RNA was purified from B4B8 cells treated in vitro with DMSO or AZD8931 (100 nM) for 1, 2 or 3 days and submitted for RNAseq. The resulting data were analyzed with GSEA and the Hallmark Pathways. b B4B8 cells were treated for 1–9 days with DMSO or AZD8931 (100 nM) and RNA was purified and submitted to RT-QPCR for the indicated IFN pathway genes. c GSEA analysis of RNAseq from B4B8 tumors treated in vivo with diluent or AZD8931 (50 mg/kg) for 3 (n = 2), 7 (n = 5) and 14 (n = 4) days. The heatmap presents the enrichment or de-enrichment of the Hallmark pathways that exhibited the highest or lowest significant (P ≤ 0.05) enrichment scores