Fig. 1

Gefitinib treatment transcriptionally induces interferon response hallmark pathways in human HNSCC cell lines. Total RNA was prepared from HN31 and UMSCC25 HNSCC cell lines treated in duplicate with DMSO or gefitinib (100 nM) for 4 days and submitted to Affymetrix Genechip analysis. a The resulting data were submitted to GSEA analysis using the Hallmark pathways and the enrichment scores for the two cell lines are presented as a heatmap. b The enrichment plots for the top enriched Hallmark pathway, the IFNα response, and the Hallmark pathway that exhibited the most negative enrichment in the gefitinib-treated samples, the E2F targets, are shown. c Selected individual genes from duplicate samples within the IFNα Hallmark pathway are presented as heatmaps with a rainbow scale depicting increased expression in response to gefitinib treatment