Fig. 3

AFMSC treatment induces molecular change in diabetic wound bed. a Representative H&E tissue sections of diabetic mouse wounds from post-op day 7. Black dashed line indicates the epithelial gap. *p < 0.05. b Quantification of epithelial gaps, n ≥ 3. c Relative gene expression of wound healing factors, with mouse models and treatments as indicated at post-op day 7. *p < 0.05. ****p < 0.0001. ns, not significant, n ≥ 3. d Relative gene expression of IL6. *p < 0.05. **p < 0.01, ns, not significant, n ≥ 3. e Representative images of immunodetection of F4/80 and Arg1 expressing cells in diabetic wound tissues with treatments as indicated. Tissues are counterstained with Ho33342. White arrows, F4/80⁺/Arg1⁺ cells. Asterisk, autofluorescence from erythrocytes