Fig. 5

Linearity of the method. a Illustration of OCR levels (up) and ECAR levels (down) versus time at baseline, after oligomycin and after FCCP addition, for cell concentration of 25,000 (very light grey line), 50,000 (light grey line), 100,000 (middle grey line), 150,000 (dark grey line) and 200,000 (black line) cells/well, 6 technical replicates per condition, each point corresponds to mean ± SD of replicates values. b Illustration of OCR levels (up) or ECAR levels (down) versus cell/well concentration at baseline (left), after oligomycin addition (middle) and FCCP addition (right). Each point represents the mean of 6 replicates values and 3 step measurements. The regression line (disrupted black line) is derived from sum of minus squares method and its metrics (equation and r² value) are represented