Fig. 4
From: Validation of a method evaluating T cell metabolic potential in compliance with ICH Q2 (R1)
Precision of the method. a Repeatability assessment of OCR detection. Left: illustration of mean ± SD of OCR levels (10 technical replicates, 3 times of measurement) at baseline (light grey bar), after oligomycin (dark grey bar) and after FCCP addition (black bar) (left). Right: table of statistical metrics at baseline, after oligomycin and after FCCP addition. CV: coefficient of variation, expressed in percentage; CI 95%: confidence interval. b Repeatability assessment of ECAR detection. Left: illustration of mean ± SD of ECAR levels (10 technical replicates, three times of measurement) at baseline (light grey bar), after oligomycin (dark grey bar) and after FCCP addition (black bar).Right: table of statistical metrics at baseline, after oligomycin and after FCCP addition. CV: coefficient of variation, expressed in percentage; CI 95%: confidence interval. c Intermediate precision assessment of OCR detection. Left: illustration of mean ± SD of OCR levels (4 independent experiments for baseline and oligomycin addition, 3 independent experiments for FCCP addition) at baseline (light grey bar), after oligomycin (dark grey bar) and after FCCP addition (black bar).Right: table of statistical metrics at baseline, after oligomycin and after FCCP addition. CV: coefficient of variation, expressed in percentage; CI 95%: confidence interval. d Intermediate precision assessment of ECAR detection. Left: illustration of mean ± SD of ECAR levels (4 independent experiments for baseline and oligomycin addition, 3 independent experiments for FCCP addition) at baseline (light grey bar), after oligomycin (dark grey bar) and after FCCP addition (black bar). Right: table of statistical metrics at baseline, after oligomycin and after FCCP addition. CV: coefficient of variation, expressed in percentage; CI 95%: confidence interval