Fig. 2

Determination of optimal JURKAT cell line concentration and specificity of the method. a Optical images of JURKAT cell confluence. JURKAT cells were plated at 50,000, 100,000 or 150,000 cells/well in a 96-well plate and photographed after poly-D-lysine cell attachment. b Ratio of cell area on cell area obtained in the 50,000 cells/well plated condition (ten area measurements per cell seeding condition). c Illustration of OCR levels versus time at baseline, after oligomycin and after FCCP addition, for cell concentration of 50,000 (light grey line), 100,000 (dark grey line) and 150,000 (black line) cells/well, 6 technical replicates per condition, each point corresponds to mean ± standard deviation (SD) of replicates values, this is one experiment representative of 4 independent experiments. d Illustration of ECAR levels versus time at baseline, after oligomycin and after FCCP addition, for cell concentration of 50,000 (light grey line), 100,000 (dark grey line) and 150,000 (black line) cells/well, 6 technical replicates per condition, each point corresponds to mean ± SD of replicates values, this is one experiment representative of 4 independent experiments