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Table 2 DNA integrity: distribution of peaks 1 and 2 (A: MagNA Pure 24 System, B: IDEAL, C: LabTurbo 24 AB; D: Chemagic 360D)

From: Qualitative and quantitative comparison of cell-free DNA and cell-free fetal DNA isolation by four (semi-)automated extraction methods: impact in two clinical applications: chimerism quantification and noninvasive prenatal diagnosis

Isolation method

A

B

C

D

75–239 bp

 Mean

90%

79%

73%

71%

 SD

9%

7%

9%

8%

 CV

10%

9%

13%

12%

 Min–Max

79–100%

68–85%

61–84%

62–84%

 P

0.0789

   

 p B–C–D

 

0.32

p240–369 bp

 Mean

5%

15%

15%

15%

 SD

5%

3%

3%

3%

 CV

99%

18%

19%

18%

 Min–Max

0–10%

11–17%

10–17%

11–18%

 P

< 0.005

 p B–C–D

 

0.865

370–579 bp

 Mean

1%

3%

6%

7%

 SD

2%

3%

2%

3%

 CV

148%

114%

40%

47%

 Min–Max

0–5%

1–8%

3–9%

2–10%

 P

0.028

 p B–C–D

 

0.122

580–1649 bp

 Mean

3%

4%

6%

7%

 SD

3%

2%

4%

3%

 CV

83%

54%

68%

41%

 Min–Max

0–7%

2–7%

2–13%

4–10%

 P

0.203

 p B–C–D

 

0.314