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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Qualitative and quantitative comparison of cell-free DNA and cell-free fetal DNA isolation by four (semi-)automated extraction methods: impact in two clinical applications: chimerism quantification and noninvasive prenatal diagnosis

Fig. 2

Cell-free DNA size profiles using the BIABooster technique. Capillary electropherogram shows the size of cfDNA isolated from a plasma. The transition from a fluorescence intensity to a concentration is done using the reference ladder. DNA concentration is given for fragment sizes between 75 and 1650 bp. Below and beyond this size range, the information given by the fluorescence intensity constitutes a quantitative indicator of the presence of small and large DNAs respectively, which cannot, to date, be converted exactly in concentration. The relative fluorescence (y-axis) of this ladder is used to calculate the size of the unknown cfDNA samples (x-axis). Thus, any deviation from the baseline, excluding the ladder, indicates the size of cfDNA. Classically, two important size peaks are identified 163 ± 1.8 pB and 307 ± 3 pB. The variations in size of the peaks and in the percentage of each fragment of cfDNA under these peaks come from the study of 120 healthy subjects after cfDNA isolation using IDEAL method (ID-solution®)

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