Fig. 3

Combined cell-therapy with MDSCs and Treg attenuate the severity of aGVHD. a Schematic representation of the development of acute GVHD after allogeneic BMT. Splenocytes (5 × 10⁶) plus bone marrow cells (5 × 10⁶) from Balb/c (syngeneic, n = 3) or B6 mice (allogeneic, n = 15) were transplanted into irradiated B/c mice (recipient, n = 25) via intra-vein injection. After BMT, recipients were divided into 5 groups (n = 10 per group) followed by IV injection with MDSCs (1 × 10⁶) and Tregs (1 × 10⁶) individually or in combination (MDSC + Treg, MT) on days 1, 7. b Weight, weight change, clinical score were monitored in mice with aGVHD. Combined data from 2 independent experiments are displayed. c Histopathological analysis of the small intestine, skin, liver and lung at 28 days after BMT. The sections were stained with hematoxylin and eosin (Intestine, liver:original magnification, × 40, skin, lung:original magnification, × 100). d The lower panel (original magnification, × 200) is the zoom-in image in the arrow region of the left panel. e Bar represent the average the histological scores of the small intestine, skin, liver and lung. Data are representative of 2 independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001 versus control (vehicle) GVHD)