Fig. 5
miR-424 from MSC-derived EVs inhibits the proliferation, migration and tube formation of HUVECs. The HUVECs used for the following detections were treated with MSC-derived EVs, EVs-depleted MSCs, MSC-derived EVs after treatments of miR-424 mimic or miR-424 inhibitor as well as their NCs. a The proliferation of HUVECs after different treatments detected by EdU assay. b The migration of HUVECs after different treatments examined by Transwell assay. c The tube formation of HUVECs after different treatments determined by Matrige-based tube formation assay. d The protein expression of VEGF and VEGFR after different treatments detected by Western blot analysis. *p < 0.05 compared with HUVECs treated with MSC-derived EVs, #p < 0.05 compared with HUVECs treated with MSC-derived EVs-mimic-NC; &p < 0.05 compared with HUVECs treated with MSC-derived EVs-inhibitor-NC. The data above were measurement data and were presented as sample mean ± standard deviation. Data among multiple groups were compared by one-way ANOVA. The cell experiment was repeated 3 times independently